Diagnostic Efficacy of Molecular Diagnostic Method for AMR in ABOiKT

  • STATUS
    Recruiting
  • End date
    Feb 28, 2022
  • participants needed
    30
  • sponsor
    Seoul National University Hospital
Updated on 25 January 2021

Summary

The purpose of this study is to determine whether the peripheral blood marker-based molecular diagnostic method developed in the previous study can differentiate and predict accommodation and antibody-mediated rejection (AMR) in ABO blood type incompatible kidney transplant (ABOiKT) patients who are prospectively recruited.

Description

Our research team retrospectively analyzes biomarkers that distinguish accommodation and acute antibody-mediated rejection through transcriptomic analysis of peripheral blood in patients with ABO blood type incompatibility kidney transplantation at Seoul National University Hospital. Through this, a classification model was developed to distinguish accommodation and antibody-mediated rejection using peripheral blood marker at the time of the protocol biopsy on the 10th day after renal transplantation, and recently reported as a new peripheral blood marker-based molecular diagnostic method. On the 10th day after surgery, transcriptomic analysis through RNA-seq was performed using samples of peripheral blood from 18 patients with accommodation and 10 patients with antibody-mediated rejection confirmed by protocol biopsy. The candidate genes were validated in the discovery set and another independent training set through quantitative real-time polymerase chain reaction (PCR). Finally, a 5-gene classification model including COX7A2L, CD69, CD14, CFD, and FOXJ3 was developed through logistic regression analysis.

To evaluate the diagnostic effectiveness (sensitivity, specificity) for the peripheral blood marker-based molecular diagnostic method by prospectively recruiting patients with ABO blood type incompatible kidney transplantation is needed. And, it is necessary to evaluate the effectiveness of whether the peripheral blood marker-based molecular diagnostic method can predict antibody-mediated rejection or accommodation as a result of protocol biopsy that will be performed on the 10th day after transplantation - peripheral blood samples collected earlier than the 10th day (day 5 and day 7) after ABO blood type incompatible kidney transplantation.

Details
Condition Antibody Mediated Rejection, ABO Blood Type Incompatible Kidney Transplantation
Treatment 5-gene classification model
Clinical Study IdentifierNCT04541914
SponsorSeoul National University Hospital
Last Modified on25 January 2021

Eligibility

Yes No Not Sure

Inclusion Criteria

Is your age greater than or equal to 19 yrs?
Gender: Male or Female
Do you have any of these conditions: Antibody Mediated Rejection or ABO Blood Type Incompatible Kidney Transplantation?
Do you have any of these conditions: ABO Blood Type Incompatible Kidney Transplantation or Antibody Mediated Rejection?
ABO blood group incompatible kidney transplant patients
Patients who consent to written consent
Patients over 19 years of age

Exclusion Criteria

Pregnant patients
Patients with a history of drug abuse within the last 6 months
Psychiatric disease history (major depression, bipolar disorder, schizophrenia, etc.)
Patients who do not want to participate in this study
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