Screening Microorganism of Cryptogenic Mechanical Pneumonia Through Next Generation Sequencing to Lung Tissue Fluid

  • End date
    Dec 25, 2024
  • participants needed
  • sponsor
    Shanghai Pulmonary Hospital, Shanghai, China
Updated on 25 January 2021


The etiology of cryptogenic organizing pneumonia (COP)was not clear, but previous studies have shown that in some patients, some pathogen could be detected in bronchoalveolar lavage fluid (BALF), and may be one of the causes of COP. This study aimed to screen the pathogenic microorganisms in BALF and lung puncture fluid of the patients with COP through the next-generation sequencing to further clarify the correlation between the incidence of COP and pathogenic microorganisms.


The cause of cryptogenic organizing pneumonia (COP) was unknown, but studies had shown that certain bacteria, viruses, or specific pathogens could be detected in bronchoalveolar lavage fluid (BALF) in some patients, and these pathogenic microorganisms may be the cause of COP. Specimens were obtained by conventional methods (sputum, blood, BALF or lung puncture fluid) for microbiological detection. Due to the influence of the quality of acquired specimens, specimen preservation and transportation which were influenced by external factors, many patients did not get good test results, which affected the diagnosis and subsequent treatment of patients. Next-generation sequencing (NGS) which could determine hundreds of thousands to millions of DNA molecules in parallel at one time, and efficiently sequence or fragment of pathogenic microorganisms. NGS had greatly improved the detection rate of pathogenic microorganisms. This study aimed to screen pathogenic microorganisms in BALF and lung puncture fluid of COP patients by NGS, and further clarify the correlation between occurrence of COP and pathogenic microorganisms.

Condition Cryptogenic Organizing Pneumonia, Idiopathic interstitial pneumonia
Treatment Next-generation sequencing
Clinical Study IdentifierNCT04511884
SponsorShanghai Pulmonary Hospital, Shanghai, China
Last Modified on25 January 2021


Yes No Not Sure

Inclusion Criteria

The patient was older than 18 years old
The patient was organizing pneumonia (OP) through pathology
The patient was diagnosed as OP from clear cause through routine detection
Chest CT shows consolidation or ground glass

Exclusion Criteria

The patient has specific characteristics of connective tissue disease or autoimmune disease
The patient has a clear other cause of OP
The patient has severe systemic diseases of heart, brain, liver, kidney and so on, and cannot tolerate invasive examination
The patient disapproves of invasive testing
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