Last updated on May 2018

Adjunctive Steps for Disinfection of Teeth With Primary Endodontic Infections

Brief description of study

This study aim to evaluate an endodontic treatment protocol using adjunctive steps for disinfection of teeth with primary endodontic infections.

Detailed Study Description

The success of endodontic treatment in teeth with necrotic pulp and apical periodontitis depends on the reduction of viable microorganisms of root canals by endodontic disinfection procedures. This study aims to evaluate by molecular methods the effect of different disinfection procedures on reduction, diversity and microbial metabolic activity. Patients with necrotic pulps and apical periodontitis in single-rooted teeth will be selected. Microbiological samples will be taken from root canals after access cavity (S1), after chemomechanical preparation with Reciproc System and 2.5% NaOCl (S2), after irrigant activation using the XP-Endo Finisher instrument (S3a) and ultrasonic activation (S3b); and after intracanal medication with calcium hydroxide for 14 days (S4). DNA and RNA will be extracted from root canal samples, and cDNA synthetized using reverse transcription reaction. The effect of treatment protocols on total microbiota will be determined by rDNA-based qPCR using universal primers for Bacteria domain. RNA of root canal samples will be used to determine the diversity of metabolically active microbiota by Reverse Transcriptase Reaction (RT), followed by amplification (PCR) and high throughput sequencing of the hypervariable region V1-V2-V3 of 16S rRNA gene. The metabolic activity of the most prevalent species / taxa will be calculated by rRNA- and rDNA-based molecular methods. The data will be analyzed by statistical tests, with 5% significance level.

Clinical Study Identifier: NCT03537664

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