Last updated on February 2018

ALLoreactive T-Cell receptOr RePertoire in kidnEy tranSplantation

Brief description of study

In this study, we will establish a workflow to generate unique patterns of the donor-reactive T cell repertoire using mixed lymphocyte reactions to select alloreactive T cell clones prior to transplantation Tissue infiltrating as well as blood bound T cells will be characterized based on:

  1. Identification of donor-specific T cell receptor sequences pre- and post-transplant by in vitro expansion to determine unique patterns
  2. Quantification and comparison of donor-specific T cell clones in kidney biopsy and blood samples.
  3. Analysis of the TCR repertoire diversities derived from kidney biopsy and blood samples and association of repertoire diversities with the histomorphological phenotype of T cell mediated rejection.
  4. Identification of T cell subtypes within the donor-reactive population. We specifically hypothesize that highly expanded donor-reactive T cell clones in both kidney tissue and blood samples at time of indication biopsy are associated with the histological phenotype of acute T cell mediated rejection. We have previously shown that there is a strong correlation between highly expanded tissue-resident T cell clones and the repertoire found in periphery blood samples. To trace and quantify donor reactive T cells we will apply a truly quantitative approach for immune repertoire profiling based on high- throughput sequencing. Our ultimate goal is to develop a diagnostic tool to assess alloreactive cellular immunoresponses based on peripheral blood samples.

Clinical Study Identifier: NCT03422224

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