Activated T-Cells Expressing 2nd or 3rd Generation CD19-Specific CAR, Advanced B-Cell NHL, ALL, and CLL (SAGAN) (SAGAN)

  • STATUS
    Recruiting
  • End date
    Feb 21, 2034
  • participants needed
    64
  • sponsor
    Baylor College of Medicine
Updated on 21 March 2022
cancer
lymphoid leukemia
fludarabine
cyclophosphamide
chronic lymphocytic leukemia
lymphoma
hodgkin's disease
flow cytometry
cell transplantation
leukemia
oximetry
tumor cells
b-cell lymphoma
lymphoma cells
lymphoma cell count

Summary

Subjects on this study have a type of lymph gland cancer called Non-Hodgkin Lymphoma, acute lymphocytic leukemia, or chronic Lymphocytic Leukemia (these diseases will be referred to as "lymphoma" or "leukemia"). The lymphoma or leukemia has come back or has not gone away after treatment.

The body has different ways of fighting infection and disease. No one way seems perfect for fighting cancers. This research study combines two different ways of fighting disease, antibodies and T cells, hoping that they will work together. Both antibodies and T cells have been used to treat patients with cancer. They have shown promise, but have not been strong enough to cure most patients.

T cells can kill tumor cells but normally there are not enough of them to kill all the tumor cells. Some researchers have taken T cells from a person's blood, grown more of them in the laboratory and then given them back to the person.

The antibody used in this study is called anti-CD19. It first came from mice that have developed immunity to human lymphoma. This antibody sticks to lymphoma cells because of a substance on the outside of these cells called CD19. CD19 antibodies have been used to treat people with lymphoma and leukemia. For this study, anti-CD19 has been changed so that instead of floating free in the blood it is now joined to the T cells. When an antibody is joined to a T cell in this way it is called a chimeric receptor.

In the laboratory, the investigators found that T cells work better if they also add proteins that stimulate T cells, such as one called CD28. Adding the CD28 makes the cells last longer in the body but not long enough for them to be able to kill the lymphoma cells. The investigators believe that if they add an extra stimulating protein, called CD137, the cells will have a better chance of killing the lymphoma cells.

The investigators are going to see if this is true by putting the CD19 chimeric receptor with CD28 alone into half of the cells and the CD19 chimeric receptor with CD28 and CD137 into the other half of the cells. These CD19 chimeric receptor T cells with CD28 and with or without CD137 are investigational products not approved by the FDA.

The purpose of this study is to find the biggest dose of chimeric T cells that is safe, to see how long the T cell with each sort of chimeric receptor lasts, to learn what the side effects are and to see whether this therapy might help people with lymphoma or leukemia.

Description

Patients will give the investigators blood to make CD19 CD28 (with and without CD137) chimeric receptor-T cells in the laboratory. These cells will be grown and frozen. To make the T cells, investigators will take blood (or blood from a donor) and stimulate it with growth factors to make the T cells grow. To get the CD19 antibody and CD28 (with or without CD137) to attach to the surface of the T cell, they will insert the antibody gene into the T cell. This is done with a virus called a retrovirus that has been made for this study and will carry the antibody gene into the T cell. This virus also helps to find the T cells in the blood after injecting them; in order to tell them apart investigators have made two viruses that are slightly different because one has CD137. These two viruses can be told apart by a special laboratory test. Because the patient will receive cells with a new gene in them, the patient will be followed for a total of 15 years to see if there are any long term side effects of gene transfer. If the patient cannot visit the clinic, he or she will be contacted by the research coordinator or physician.

When subjects enroll on this study, they will be assigned a dose of CD19 chimeric receptor-T cells. Several studies suggest that the infused T cells need room to be able to proliferate and accomplish their functions and that this may not happen if there are too many other T cells in circulation.

Because of that, if the subject's level of circulating T cells is relatively high, s/he may receive one treatment of cyclophosphamide (Cytoxan) and fludarabine if the doctor thinks this is appropriate. This drug will decrease the numbers of the subject's own T cells before infusion of the CD19 chimeric receptor T cells. If subject is already receiving chemotherapy, this may not be needed. The investigators would prefer subjects do not receive other chemotherapy until 6 weeks after cell infusion but they can do so if their doctor thinks it is medically necessary.

Patients will be given an injection of cells into the vein through an IV at the assigned dose. The injection will take about 20 minutes. The investigators will follow them in the clinic after the injection for up to 3 hours.

If after a 6 week evaluation period after the infusion, the patient seems to be experiencing a benefit (confirmed by radiological studies, physical exam and/or symptoms), s/he may be able to receive up to five additional doses of the T cells if s/he wishes. The first repeat infusion can only take place at least 6 weeks after the first infusion. Any additional infusions after that would be at least 4 weeks apart. All additional infusions will be at the same dose level received the first time or a lower dose. The treatment will be given by the Center for Cell and Gene Therapy at Texas Children's Hospital or Houston Methodist Hospital.

Details
Condition Non-Hodgkin Lymphoma, Chronic Lymphocytic Leukemia, Acute Lymphocytic Leukemia
Treatment cyclophosphamide, Fludarabine, CD19 CAR T Cells
Clinical Study IdentifierNCT01853631
SponsorBaylor College of Medicine
Last Modified on21 March 2022

Eligibility

Yes No Not Sure

Inclusion Criteria

PROCUREMENT
Referred patients (or respective donors) will initially be consented for procurement of
blood for generation of the transduced ATL. Eligibility criteria at this stage include
Diagnosis of recurrent B-cell lymphoma or leukemia (ALL or CLL), or newly diagnosed
patients unable to receive or complete standard therapy OR diagnosis of
CD19-positive tumor (result can be pending at this time)
relapsed/refractory aggressive B-cell lymphoma with a treatment plan that will include
high dose therapy and autologous stem cell transplantation
Hgb greater than or equal to 7.0 (can be a transfused value)
If pheresis required to collect blood
Age <= 75 years. The first 3 patients treated on the study should be adults (>= 18
Creatinine < 1.5 x upper limit normal
years)
TREATMENT
AST <1.5 × upper limit normal
PT and APTT <1.5 × upper limit normal
Informed consent explained to, understood by and signed by patient/guardian (and
CD19-positive tumor
donor, where applicable). Patient/guardian given copy of informed consent
Bilirubin less than 3 times the upper limit of normal
Diagnosis of recurrent B-cell lymphoma leukemia (ALL or CLL), or newly diagnosed
AST less than 5 times the upper limit of normal
patients unable to receive or complete standard therapy OR diagnosis of
Estimated GFR > 50 mL/min
relapsed/refractory aggressive B-cell lymphoma with a treatment plan that will include
Pulse oximetry of > 90% on room air
high dose therapy and autologous stem cell transplantation
Karnofsky or Lansky score of > 60%
Age <= 75 years. The first 3 patients treated on the study should be adults (>= 18
Life expectancy of greater than 12 weeks
years)
Recovered from acute toxic effects of prior chemotherapy at least one week before
entering this study. PD1/PDL1 inhibitors will be allowed if medically indicated
Available autologous or syngeneic activated peripheral blood T cell products (CD28ζ
and CD28/CD137ζ) with more than or equal to 15% expression of CD19.CAR determined by
flow cytometry
Sexually active patients must be willing to utilize one of the more effective birth
control methods during the study and for 6 months after the study is concluded. The
male partner should use a condom
Patients or legal guardians must sign an informed consent indicating that they are
aware this is a research study and have been told of its possible benefits and toxic
side effects. Patients or their guardians will be given a copy of the consent form

Exclusion Criteria

PROCUREMENT
Active infection requiring antibiotics
TREATMENT
History of hypersensitivity reactions to murine protein-containing products
Pregnant or lactating
Tumor in a location where enlargement could cause airway obstruction
Active infection with HIV or HTLV
No history of other cancer (except non-melanoma skin cancer or in situ breast cancer
or cervix cancer) unless the tumor was successfully treated with curative intent at
least 2 years before trial entry
Currently receiving any investigational agents or received any tumor vaccines within
the previous 6 weeks. (Note treatment with PD1/PDL1 inhibitors is allowed.)
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