Last updated on February 2018

The Effects of Nanocurcumin on Serum miRNA and Th17 Cells Development Factors in Ankylosing Spondylitis Patients


Brief description of study

Ankylosing spondylitis is a chronic autoimmune disease in which T cells associated with the disorder. The disease often affects the spine and hip bones. Inflammation involved in the area of the sacrum - Sacroiliac hip joints, pelvis, spinal lumbar, thoracic and cervical. Curcumin is a polyphenol. It is an active component of turmeric which is a perennial plant. Curcumin binding with a range of protein and the have ability to inhibit the activity of various kinases. Curcumin has a combination of activities such as antioxidant, anti-proliferation, anti-invasive, and can used in the treatment of Alzheimer's, Parkinson's, Multiple sclerosis, Cardiovascular disease, Bacterial diseases and Arthritis and ankylosing spondylitis. This increases the solubility of curcumin in nanomicelles spherical water to more than 100 thousand times, which significantly enhances the absorption of curcumin. A group of circulating miRNA in plasma is found to be the change they can be involved in inflammation or inhibit it. MiRNA expression profiles in blood and serum samples taken from patients with ankylosing spondylitis review that is miRNA-326 also affects cells of the immune system may be involved in disease ankylosing spondylitis. MiRNA-326 induces differentiation of Th17 cells via specific transcription factors, such as the RoRt. MiRNA326 increased the expression of transcription factor RoRt and Th17 cell differentiation TCD4 + cells resulting in the production of inflammatory cytokine IL17 increased inflammation and progression of inflammatory disease ankylosing spondylitis (AS).

Detailed Study Description

This study is a randomized double-blind placebo control clinical trial conducted on two groups for 4 months. Patients receive oral nanocurcumin (80 mg / day) as compared with placebo group. After sampling both groups and separating plasma, peripheral blood mononuclear cells isolated by using ficoll and fresh and cultured in the presence of PHA. PBMC is used to measure the levels of miRNA-326, IL-17 and RORt expression. the frequency of Th17 cells in peripheral blood will be examined by flowcytometry. To check the amount of the cytokine IL-17 in cultured cells supernatant is used ELISA technique.

Clinical Study Identifier: NCT03140657

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Sanam Dolati, Ph.D

Connective Tissue Diseases Research Center
Tabriz, Iran, Islamic Republic of
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Sanam Dolati, Ph.D

SCARM institiute
Tabriz, Iran, Islamic Republic of
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